PP1C, Protein Phosphatase 1
Source: Rabbit skeletal muscle
Purity: > 90% by SDS-PAGE, apparent Mr ~ 36-kDa
Supplied: 1 μg in 50 μl 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA and 50% glycerol.
Activity: ~ 2000 units/mg with 32P-labeled phosphorylase as substrate. One unit is the amount of PP1C that releases 1 nmol of inorganic phosphate from 32P-labeled phosphorylase per min. Maintain preparations in aliquots at -70° C. Avoid repeated thawing.
Synonyms: Catalytic Subunit of Protein Phosphatase 1; Protein Phosphatase 1C; PP1C
Figure: SDS-PAGE of a sample of a purified preparation of PP1C. The gel was stained with Coomassie Blue.
In contrast to widely available recombinant forms of the enzyme, PP1C isolated from rabbit skeletal muscle and other tissue sources is divalent cation-independent. Its activity is nonetheless stimulated by Mn2+ with several substrates.
Background: Protein phosphatase 1 (PP1) is one of the four major cytoplasmic protein serine/threonine phosphatases present in all mammalian cells. It is highly conserved and has been found to play a key role in the cell cycle and cell proliferation activities. The enzyme is a growth factor-stimulated divalent cation independent phosphatase. It is inhibited by a number of toxins including mycrocyctins, calyculins (clavosine A and B), nodularins, and okadaic acid. The enzyme is phosphorylated on its catalytic subunit at Thr320 by cdc2 kinase, resulting in inhibition of the phosphatase.
References: Biochem J 287: 1019; Proc Natl Acad Sci USA 90: 2500
GloboZymes抗PAK
GloboZymes Anti-PAK是一种亲和纯化的多克隆兔抗体。它被生产为对应于人PAK1残基484-502的合成肽。该抗体可用于蛋白质印迹。例如，抗体可以与30μgHEK293细胞提取物以1：1000稀释度使用。除了PAK1，该抗体还显示出与PAK2的反应性。
同义词：Anti-p65PAK; 抗p68PAK; p21活化蛋白激酶抗体。
用Ani-PAK进行的HEK293提取物的Western印迹
图：将HEK293细胞提取物（30μg）用GloboZymes亲和纯化的Anti-PAK多克隆抗体进行Western印迹。该频带对应于PAK65（65 kDa）。
背景：p21-GTPase激活的蛋白激酶家族（PAK's）调节许多过程。这些过程包括细胞形态和细胞骨架组织，细胞运动，凋亡，神经元功能和细胞周期进程等。PAK与几种重要的调节蛋白相互作用，包括蛋白磷酸酶2A（PP2A），核糖体蛋白S6激酶，混合谱系激酶（MLK2）和SH3-SH2接头Nck。PAK的生理学靶标包括p38 MAP激酶，JNK，cRaf-1和SynapsinI。PAK的催化结构域通过半胱天冬酶依赖性途径在凋亡细胞中产生。与天然酶不同，催化域不受Rac或Cdc42的影响。
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