PP2A1, Protein Phosphatase 2A1
Source: Bovine kidney
Purity: > 90% by SDS-PAGE, subunit apparent Mr’s ~ 36-, 55- and 65-kDa
Supplied: In 50 μl 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA and 50% glycerol
Activity: ~ 2000 units/mg with Myelin Basic Protein (MBP) as substrate. One unit is the amount of PP2A1 that releases 1 nmol of inorganic phosphate from 32P-labeled MBP per min. Maintain preparations in aliquots at -70° C. Avoid repeated thawing.
Synonyms: Protein Phosphatase 2A1; PP2A1
GloboZymes PP2A1 preparations represent homogenous preparations of a heterotrimeric form of the phosphatase. These preparations are composed of A/PR65 , 55-kDa B and 36-kDa catalytic subunits. They are used to study enzyme kinetics and regulation, to dephosphorylate target substrates, and to evaluate the effects of test substances on the activity of the phosphatase.
Figure: SDS-PAGE pattern of purified preparation of PP2A1 showing, from top to bottom, the A/PR65 65-kDa, 55-kDa B and 36-kDa catalytic subunits. The gel was stained with Coomassie Blue.
Background: Protein phosphatase 2A (PP2A) is a growth and tumor suppressor. It is a multifunctional divalent cation-independent protein serine/threonine phosphatase involved in regulating numerous cellular processes, including the cell cycle, growth and differentiation. Physiological targets of PP2A include cell surface receptors and ion channels, and protein kinases involved in mitogenic signaling and the cell cycle. They also include transcription factors and key regulatory enzymes involved in metabolism. The tumor promoter okadaic acid potently inhibits PP2A. The phosphatase is also inhibited potently by two cancer-associated cellular proteins, I1PP2A and I2PP2A. During viral transformation, the SV40 small t antigen replaces the B subunit and subverts the physiological function of a subset of heterotrimeric PP2A complexes in a substrate-selective manner.
References: Biochem J 287, 1019-1022; Proc Natl Acad Sci USA 90, 2500-2504; Trends Cell Biol 4, 287-291; Ann Rev Cell Biol 10, 55-86; Biochem J 353, 417
GloboZymes抗PAK
GloboZymes Anti-PAK是一种亲和纯化的多克隆兔抗体。它被生产为对应于人PAK1残基484-502的合成肽。该抗体可用于蛋白质印迹。例如，抗体可以与30μgHEK293细胞提取物以1：1000稀释度使用。除了PAK1，该抗体还显示出与PAK2的反应性。
同义词：Anti-p65PAK; 抗p68PAK; p21活化蛋白激酶抗体。
用Ani-PAK进行的HEK293提取物的Western印迹
图：将HEK293细胞提取物（30μg）用GloboZymes亲和纯化的Anti-PAK多克隆抗体进行Western印迹。该频带对应于PAK65（65 kDa）。
背景：p21-GTPase激活的蛋白激酶家族（PAK's）调节许多过程。这些过程包括细胞形态和细胞骨架组织，细胞运动，凋亡，神经元功能和细胞周期进程等。PAK与几种重要的调节蛋白相互作用，包括蛋白磷酸酶2A（PP2A），核糖体蛋白S6激酶，混合谱系激酶（MLK2）和SH3-SH2接头Nck。PAK的生理学靶标包括p38 MAP激酶，JNK，cRaf-1和SynapsinI。PAK的催化结构域通过半胱天冬酶依赖性途径在凋亡细胞中产生。与天然酶不同，催化域不受Rac或Cdc42的影响。
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