AK/PAK2 Detection Kit
GloboZymes AK/PAK2 Detection Kit includes 200 μg of affinity-purified Anti-AK/PAK2 and a sample of highly purified AK/PAK2. The AK/PAK2 sample is provided in SDS sample buffer ready for use in five applications as positive control in Western blots. The polyclonal antibody was produced in rabbits to a synthetic peptide corresponding to residues 273-291 of the catalytic domain of human lymphocyte PAK2.
Synonyms: p21-Activated Protein Kinase PAK2 Catalytic Domain Western Blot Detection Kit.
Background: The catalytic domain of PAK2 was originally isolated as an Auto-activated Protein Kinase (AK). It was so termed because the purified enzyme underwent rapid autophosphorylation and concomitant 15-30-fold activation. Subsequent identification of PAK’s and amino sequencing of AK revealed that it was equivalent to the catalytic domain of PAK2*. Unlike native PAK2, AK does not require the p21-GTPases Cdc42 and Rac for activity. AK is a physiologically relevant form of PAK2. It is produced from the native enzyme via a caspase-dependent pathway in cells undergoing apoptosis.
References: Proc Natl Acad Sci USA 90: 2500 J Biol Chem 268: 11193 J Cell Physiol 178: 397 J Biol Chem 276: 14909 * Damuni Z. unpublished observations
GloboZymes抗PAK
GloboZymes Anti-PAK是一种亲和纯化的多克隆兔抗体。它被生产为对应于人PAK1残基484-502的合成肽。该抗体可用于蛋白质印迹。例如，抗体可以与30μgHEK293细胞提取物以1：1000稀释度使用。除了PAK1，该抗体还显示出与PAK2的反应性。
同义词：Anti-p65PAK; 抗p68PAK; p21活化蛋白激酶抗体。
用Ani-PAK进行的HEK293提取物的Western印迹
图：将HEK293细胞提取物（30μg）用GloboZymes亲和纯化的Anti-PAK多克隆抗体进行Western印迹。该频带对应于PAK65（65 kDa）。
背景：p21-GTPase激活的蛋白激酶家族（PAK's）调节许多过程。这些过程包括细胞形态和细胞骨架组织，细胞运动，凋亡，神经元功能和细胞周期进程等。PAK与几种重要的调节蛋白相互作用，包括蛋白磷酸酶2A（PP2A），核糖体蛋白S6激酶，混合谱系激酶（MLK2）和SH3-SH2接头Nck。PAK的生理学靶标包括p38 MAP激酶，JNK，cRaf-1和SynapsinI。PAK的催化结构域通过半胱天冬酶依赖性途径在凋亡细胞中产生。与天然酶不同，催化域不受Rac或Cdc42的影响。
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