Anti-AK/PAK2
GloboZymes Anti-AK/PAK2 is an affinity-purified polyclonal antibody produced in rabbits to a synthetic peptide corresponding to residues 273-291 of the catalytic domain of human lymphocyte PAK2. The antibody is useful for Western blotting. For example, it can be used at 1:1000 dilution with 20 μg HEK293 cell extracts. The antibody reacts with PAK2 and its catalytic domain from diverse tissue sources.
Synonyms: Polyclonal Antibody to Auto-Activated Protein Kinase; Autophosphorylation-Activated Protein Kinase; Catalytic Domain of PAK2; Catalytic Domain of p21-Activated Protein Kinase.
Background: The catalytic domain of PAK2 (AK/PAK2) was originally isolated as an auto-activated protein kinase (AK). Thus, the purified enzyme underwent rapid autophosphorylation and concomitant 15-30-fold activation. Subsequent discovery of the PAK family of enzymes and amino sequencing of AK revealed that AK was equivalent to the catalytic domain of PAK2. Unlike native PAK2, AK does not require p21-GTPases Cdc42 and Rac for expression of its activity. Interestingly, AK is a physiologically relevant form of PAK2. It is produced from the native enzyme via a caspase-dependent pathway in cells undergoing apoptosis.
Figure: Purified preparations of AK/PAK2 were subjected to Western Blot with GloboZymes affinity-purified rabbit polyclonal antibody Anti-AK/PAK2. The band correspond to AK/PAK2 (~ 36 kDa).
References: Proc Natl Acad Sci USA 90: 2500; J Biol Chem 268: 11193; J Cell Physiol 178: 397; J Biol Chem 276: 14909
GloboZymes抗PAK
GloboZymes Anti-PAK是一种亲和纯化的多克隆兔抗体。它被生产为对应于人PAK1残基484-502的合成肽。该抗体可用于蛋白质印迹。例如，抗体可以与30μgHEK293细胞提取物以1：1000稀释度使用。除了PAK1，该抗体还显示出与PAK2的反应性。
同义词：Anti-p65PAK; 抗p68PAK; p21活化蛋白激酶抗体。
用Ani-PAK进行的HEK293提取物的Western印迹
图：将HEK293细胞提取物（30μg）用GloboZymes亲和纯化的Anti-PAK多克隆抗体进行Western印迹。该频带对应于PAK65（65 kDa）。
背景：p21-GTPase激活的蛋白激酶家族（PAK's）调节许多过程。这些过程包括细胞形态和细胞骨架组织，细胞运动，凋亡，神经元功能和细胞周期进程等。PAK与几种重要的调节蛋白相互作用，包括蛋白磷酸酶2A（PP2A），核糖体蛋白S6激酶，混合谱系激酶（MLK2）和SH3-SH2接头Nck。PAK的生理学靶标包括p38 MAP激酶，JNK，cRaf-1和SynapsinI。PAK的催化结构域通过半胱天冬酶依赖性途径在凋亡细胞中产生。与天然酶不同，催化域不受Rac或Cdc42的影响。
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