Anti-PP2AC
GloboZymes Anti-PP2AC is a polyclonal antiserum raised in rabbits to the catalytic subunit of bovine kidney protein phosphatase 2A. The antibody is useful for Western blotting. It can be used at 1:1000 dilution with 30 μg bovine liver extracts, for example.
Synonym: Polyclonal Antibody to the Catalytic Subunit of Protein Phosphatase 2A; Anti-PP2AC
Background: Protein phosphatase 2A (PP2A) is a divalent cation-independent multifunctional protein serine threonine phosphatase. It regulates numerous cellular processes including the cell cycle, growth and differentiation. Physiological targets of PP2A include cell surface receptors and ion channels, and protein kinases involved in mitogenic signaling and the cell cycle. PP2A also acts on key regulatory enzymes and proteins involved in metabolism, as well as numerous transcription factors. PP2A is a growth and tumor suppressor. It is inhibited potently by tumor promoters such as okadaic acid, and by two cancer-associated cellular proteins, I1PP2A and I2PP2A/SET. Additionally, the SV40 small t antigen replaces the B subunit of PP2A during viral transformation. This subverts the physiological function of a subset of PP2A complexes in a substrate selective manner.
References: Biochem J 287, 1019-1022; Proc Natl Acad Sci USA 90, 2500-2504; Trends Cell Biol 4, 287-291; Ann Rev Cell Biol 10, 55-86; Biochem J 353, 417
GloboZymes抗PAK
GloboZymes Anti-PAK是一种亲和纯化的多克隆兔抗体。它被生产为对应于人PAK1残基484-502的合成肽。该抗体可用于蛋白质印迹。例如，抗体可以与30μgHEK293细胞提取物以1：1000稀释度使用。除了PAK1，该抗体还显示出与PAK2的反应性。
同义词：Anti-p65PAK; 抗p68PAK; p21活化蛋白激酶抗体。
用Ani-PAK进行的HEK293提取物的Western印迹
图：将HEK293细胞提取物（30μg）用GloboZymes亲和纯化的Anti-PAK多克隆抗体进行Western印迹。该频带对应于PAK65（65 kDa）。
背景：p21-GTPase激活的蛋白激酶家族（PAK's）调节许多过程。这些过程包括细胞形态和细胞骨架组织，细胞运动，凋亡，神经元功能和细胞周期进程等。PAK与几种重要的调节蛋白相互作用，包括蛋白磷酸酶2A（PP2A），核糖体蛋白S6激酶，混合谱系激酶（MLK2）和SH3-SH2接头Nck。PAK的生理学靶标包括p38 MAP激酶，JNK，cRaf-1和SynapsinI。PAK的催化结构域通过半胱天冬酶依赖性途径在凋亡细胞中产生。与天然酶不同，催化域不受Rac或Cdc42的影响。
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