PAK Detection Kit
GloboZymes PAK Detection Kit includes a 200 μg aliquot of affinity-purified Anti-PAK and a sample of highly purified PAK1 . The PAK1 aliquot is provided in SDS sample buffer ready for use in five applications as positive control in Western blots.
The antibody was produced in rabbits to a synthetic peptide corresponding to residues 484-502 of human PAK1. The polyclonal antibody is useful for Western blotting. For example, it is used at 1:1000 dilution with 30 μg HEK293 cell extracts . In addition to PAK1, the antibody displays reactivity with PAK2.
Synonyms: PAK, p21-Activated Protein Kinase, p65PAK, p68PAK Western Blot Detection Kit
Figure: An extract of HEK293 cells was subjected to Western blotting with GloboZymes affinity-purified Anti-PAK polyclonal antibody. The arrow denotes the position of PAK65 (65 kDa).
Background: The p21-GTPase-activated protein kinases (PAK’s) are a family of enzymes that regulate numerous processes. These processes include cell morphology and cytoskeleton organization, cell motility, apoptosis, neuronal functions, cell cycle progression, viral replication and other processes. PAK’s interact with several important regulatory proteins including protein phosphatase 2A (PP2A), ribosomal protein S6 kinase, Mixed Lineage Kinase (MLK2), and the SH3-SH2 adapter Nck. Physiological targets of PAK include p38 MAP kinase, JNK, cRaf-1 and Synapsin I. The catalytic domain of PAK is produced in apoptotic cells via a caspase-dependent pathway. Unlike the native enzymes, the catalytic domain does not require Rac or Cdc42 to undergo autophosphorylation and activation.
References: Nature 367: 40; J Biol Chem 270: 21695
GloboZymes抗PAK
GloboZymes Anti-PAK是一种亲和纯化的多克隆兔抗体。它被生产为对应于人PAK1残基484-502的合成肽。该抗体可用于蛋白质印迹。例如，抗体可以与30μgHEK293细胞提取物以1：1000稀释度使用。除了PAK1，该抗体还显示出与PAK2的反应性。
同义词：Anti-p65PAK; 抗p68PAK; p21活化蛋白激酶抗体。
用Ani-PAK进行的HEK293提取物的Western印迹
图：将HEK293细胞提取物（30μg）用GloboZymes亲和纯化的Anti-PAK多克隆抗体进行Western印迹。该频带对应于PAK65（65 kDa）。
背景：p21-GTPase激活的蛋白激酶家族（PAK's）调节许多过程。这些过程包括细胞形态和细胞骨架组织，细胞运动，凋亡，神经元功能和细胞周期进程等。PAK与几种重要的调节蛋白相互作用，包括蛋白磷酸酶2A（PP2A），核糖体蛋白S6激酶，混合谱系激酶（MLK2）和SH3-SH2接头Nck。PAK的生理学靶标包括p38 MAP激酶，JNK，cRaf-1和SynapsinI。PAK的催化结构域通过半胱天冬酶依赖性途径在凋亡细胞中产生。与天然酶不同，催化域不受Rac或Cdc42的影响。
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