eIF4E(S53A)
Source: Recombinant human produced in E. coli
Purity: > 90% by SDS-PAGE, apparent Mr ~ 28-kDa
Supplied: In 50 μl of 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA and 10% glycerol. Maintain in aliquots at -70° C. Avoid repeated thawing.
Synonym: Eukaryotic Protein Synthesis Initiation Factor 4E Serine 53 to Alanine Mutant; eIF-4ES53A.
Background: The mRNA Cap-binding protein synthesis initiation factor 4E (eIF4E) participates in an early rate limiting step in protein synthesis. Over-expression of eIF4E leads to cell transformation and tumorigenesis and occurs in a number of cancer cells. In quiescent cells, eIF4E occurs as an inactive complex with the 4EBP binding proteins. In response to insulin and other extracellular stimuli, eIF4E dissociates from 4EBP and is recruited to the active eIF4F complex. The active eIF4F complex contains eIF4A and eIF4G in addition to eIF4E. In response to insulin and other growth factors, eIF4E is phosphorylated at Ser209. This phosphorylation enhances the affinity of eIF4E to capped mRNA. It is catalyzed by the Mitogen-Activated Protein Kinase (MAPK) Interacting Kinases (Mnk’s). It is also catalyzed by an insulin-stimulated kinase termed cPK which also phosphorylates Thr210 on the initiation factor.
Figure: SDS-PAGE pattern of a purified preparation of eIF4E(S53A). The gel was stained with Coomassie Blue.
References: J Biol Chem 270, 14824-14828; J Biol Chem 270, 14597-14603; Biochime 76, 822-830; J Biol Chem 271, 11831-11837; J Biol Chem 270, 21684-21688
GloboZymes抗PAK
GloboZymes Anti-PAK是一种亲和纯化的多克隆兔抗体。它被生产为对应于人PAK1残基484-502的合成肽。该抗体可用于蛋白质印迹。例如，抗体可以与30μgHEK293细胞提取物以1：1000稀释度使用。除了PAK1，该抗体还显示出与PAK2的反应性。
同义词：Anti-p65PAK; 抗p68PAK; p21活化蛋白激酶抗体。
用Ani-PAK进行的HEK293提取物的Western印迹
图：将HEK293细胞提取物（30μg）用GloboZymes亲和纯化的Anti-PAK多克隆抗体进行Western印迹。该频带对应于PAK65（65 kDa）。
背景：p21-GTPase激活的蛋白激酶家族（PAK's）调节许多过程。这些过程包括细胞形态和细胞骨架组织，细胞运动，凋亡，神经元功能和细胞周期进程等。PAK与几种重要的调节蛋白相互作用，包括蛋白磷酸酶2A（PP2A），核糖体蛋白S6激酶，混合谱系激酶（MLK2）和SH3-SH2接头Nck。PAK的生理学靶标包括p38 MAP激酶，JNK，cRaf-1和SynapsinI。PAK的催化结构域通过半胱天冬酶依赖性途径在凋亡细胞中产生。与天然酶不同，催化域不受Rac或Cdc42的影响。
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