c-Jun GST
Source: Recombinant human produced in E. coli
Purity: > 90% by SDS-PAGE, apparent Mr ~ 68-kDa
Supplied: In 50 μl of 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA and 10% glycerol. Maintain in aliquots at -70° C. Avoid repeated thawing.
GloboZymes c-Jun preparations are suitable for studies on the kinetics, enzymology and regulation of the phosphorylation of this important proto-oncogene. The purified preparations are effective as substrate for JNK/SAPK, MAPK, GSK-3 and CK-2 among other protein kinases. The GST-tagged preparations are also used for binding and pull down studies.
Background: The proto-oncogene c-Jun is an important transactivating factor. It is a component of the AP-1 and ATF-2 transcription factors. A wide variety of cellular signals regulate c-Jun acutely via modulation of its phosphorylation state. Phosphorylation can both inhibit and activate the proto-oncogene. GSK-3 phosphorylates c-Jun at a region proximal to the highly basic DNA binding domain (Thr239/Ser243/Ser249). This phosphorylation prevents DNA association with c-Jun homodimers, but not with c-Jun-c-Fos heterodimers. In contrast, phosphorylation by JNK/SAPK at Ser63 and Ser73, in a region proximal to the transactivation domain, activates the c-Jun/AP-1 and c-Jun/ATF2 transcriptional activator complexes to induce a number of genes. The MAP kinases ERK1 and ERK2 phosphorylate c-Jun at Thr91/Thr93/Thr95. However, the significance of these phosphorylations is uncertain.
References: Al-Murrani SW et al (1999) Biochem J 341, 293-298 Karin M (1995) J Biol Chem 270, 16483-16486 Woodgett JR et al (1996) Phil Trans R Soc Lond 351, 135-141 Kyriakis JM & Avruch J (2001) Physiol Rev 81, 807-869
GloboZymes抗PAK
GloboZymes Anti-PAK是一种亲和纯化的多克隆兔抗体。它被生产为对应于人PAK1残基484-502的合成肽。该抗体可用于蛋白质印迹。例如，抗体可以与30μgHEK293细胞提取物以1：1000稀释度使用。除了PAK1，该抗体还显示出与PAK2的反应性。
同义词：Anti-p65PAK; 抗p68PAK; p21活化蛋白激酶抗体。
用Ani-PAK进行的HEK293提取物的Western印迹
图：将HEK293细胞提取物（30μg）用GloboZymes亲和纯化的Anti-PAK多克隆抗体进行Western印迹。该频带对应于PAK65（65 kDa）。
背景：p21-GTPase激活的蛋白激酶家族（PAK's）调节许多过程。这些过程包括细胞形态和细胞骨架组织，细胞运动，凋亡，神经元功能和细胞周期进程等。PAK与几种重要的调节蛋白相互作用，包括蛋白磷酸酶2A（PP2A），核糖体蛋白S6激酶，混合谱系激酶（MLK2）和SH3-SH2接头Nck。PAK的生理学靶标包括p38 MAP激酶，JNK，cRaf-1和SynapsinI。PAK的催化结构域通过半胱天冬酶依赖性途径在凋亡细胞中产生。与天然酶不同，催化域不受Rac或Cdc42的影响。
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