Pyruvate Dehydrogenase Complex (PDC)
Source: Bovine heart
Purity: > 90% by SDS-PAGE, subunit apparent Mr’s ~ 74,000 (E2), 53,000 (E3), 41,000 (E1a), 48,000 (E3-BP) and 36,000 (E1β). Apparent Mr ~ 8,500,000 by sedimentation equilibrium centrifugation.
Supplied: In 50 μl 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA and 10% glycerol. Maintain in aliquots at -70° C. Avoid repeated thawing.
Activity: ~ 12-18 μmol of NADH formed per min per mg of protein with pyruvate as substrate
Background: The pyruvate dehydrogenase complex (PDC) is a mitochondrial enzyme which catalyzes the oxidative decarboxylation of pyruvate. PDC is regulated by reversible phosphorylation in response to extracellular stimuli. A tightly associated protein kinase phosphorylates and inactivates PDC. In contrast, loosely associated mitochondiral protein phosphatases dephosphorylate and activate PDC.
References: Methods Enzymol 166: 321 Methods Enzymol 89:376
GloboZymes抗PAK
GloboZymes Anti-PAK是一种亲和纯化的多克隆兔抗体。它被生产为对应于人PAK1残基484-502的合成肽。该抗体可用于蛋白质印迹。例如，抗体可以与30μgHEK293细胞提取物以1：1000稀释度使用。除了PAK1，该抗体还显示出与PAK2的反应性。
同义词：Anti-p65PAK; 抗p68PAK; p21活化蛋白激酶抗体。
用Ani-PAK进行的HEK293提取物的Western印迹
图：将HEK293细胞提取物（30μg）用GloboZymes亲和纯化的Anti-PAK多克隆抗体进行Western印迹。该频带对应于PAK65（65 kDa）。
背景：p21-GTPase激活的蛋白激酶家族（PAK's）调节许多过程。这些过程包括细胞形态和细胞骨架组织，细胞运动，凋亡，神经元功能和细胞周期进程等。PAK与几种重要的调节蛋白相互作用，包括蛋白磷酸酶2A（PP2A），核糖体蛋白S6激酶，混合谱系激酶（MLK2）和SH3-SH2接头Nck。PAK的生理学靶标包括p38 MAP激酶，JNK，cRaf-1和SynapsinI。PAK的催化结构域通过半胱天冬酶依赖性途径在凋亡细胞中产生。与天然酶不同，催化域不受Rac或Cdc42的影响。
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