Cdc42
Cdc42 Source: GST-tagged recombinant human produced in E. coli
Purity: > 90% by SDS-PAGE, apparent Mr ~ 48-kDa.
Supplied: In 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA, 0.03% Brij-35 and 10% glycerol. 5 μg of the purified protein are supplied in 50 μl of the buffer; 25 μg or 50 μg of the purified protein are supplied in 100 μl of the buffer. Maintain preparations in aliquots at -70° C. Avoid repeated thawing.
Synonym: GST-Cdc42
GloboZymes GST-tagged Cdc42 preparations are effective for binding and pull-down affinity studies.
Background: Cdc42 is a Rho-like GTP-binding protein. It is involved in signal transduction regulation and cytoskeleton reorganization in response to growth factor stimulation. Cdc42 activates members of the PAK protein kinase family by binding to their regulatory domain. The catalytic domain is then able to undergo autophosphorylation and concomitant activation.
Figure: Purified preparation of recombinant human GST-tagged cdc42 was subjected to SDS-PAGE. The gel was stained with Coomassie Blue.
References: Nature 367: 40 J Biol Chem 270: 21695
GloboZymes抗PAK
GloboZymes Anti-PAK是一种亲和纯化的多克隆兔抗体。它被生产为对应于人PAK1残基484-502的合成肽。该抗体可用于蛋白质印迹。例如，抗体可以与30μgHEK293细胞提取物以1：1000稀释度使用。除了PAK1，该抗体还显示出与PAK2的反应性。
同义词：Anti-p65PAK; 抗p68PAK; p21活化蛋白激酶抗体。
用Ani-PAK进行的HEK293提取物的Western印迹
图：将HEK293细胞提取物（30μg）用GloboZymes亲和纯化的Anti-PAK多克隆抗体进行Western印迹。该频带对应于PAK65（65 kDa）。
背景：p21-GTPase激活的蛋白激酶家族（PAK's）调节许多过程。这些过程包括细胞形态和细胞骨架组织，细胞运动，凋亡，神经元功能和细胞周期进程等。PAK与几种重要的调节蛋白相互作用，包括蛋白磷酸酶2A（PP2A），核糖体蛋白S6激酶，混合谱系激酶（MLK2）和SH3-SH2接头Nck。PAK的生理学靶标包括p38 MAP激酶，JNK，cRaf-1和SynapsinI。PAK的催化结构域通过半胱天冬酶依赖性途径在凋亡细胞中产生。与天然酶不同，催化域不受Rac或Cdc42的影响。
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