Inhibitor-1
Source: Recombinant human produced in E. coli
Purity: > 95% by SDS-PAGE, apparent Mr ~ 27-kDa
Supplied: 1 μg in 50 μl of 50 mM Tis-HCl pH 7.0 buffer containing 0.1 mM phenylmethanesulfonyl fluoride, 14 mM β-mercaptoethanol and 10% glycerol. Maintain in aliquots at -70°C. Avoid repeated freeze thaw cycles.
Synonyms: I1; Inhibitor 1
Background: Inhibitor-1 is a 171 amino acid protein with an average molecular weight of 18.9 kDa. It is a specific inhibitor of Type-1 protein serine/threonine phosphatases (PP1). The IC50 for inhibition of PP1 is ~ 1 to 2 nm. The selectivity and potency of inhibitor-1 are conferred by its N-terminal sequence KIQF and its activation by phosphorylation at Thr35 catalyzed by protein kinase A. Dephosphorylation and inactivation can be carried out in the presence of Ca2+ by PP2B (Calcineurin).
References: Connor, J. H. et al (1998) J. Biol. Chem. 273, 27716-27724 ; Connor, J. H. et al (1999) J. Biol. Chem 274, 22366-22372; Endo, S. et al (1996) Biochemistry 35, 5220-5228 ; Bibb, J. A. et al (2001) J. Biol. Chem. 276, 14490-14497
GloboZymes抗PAK
GloboZymes Anti-PAK是一种亲和纯化的多克隆兔抗体。它被生产为对应于人PAK1残基484-502的合成肽。该抗体可用于蛋白质印迹。例如，抗体可以与30μgHEK293细胞提取物以1：1000稀释度使用。除了PAK1，该抗体还显示出与PAK2的反应性。
同义词：Anti-p65PAK; 抗p68PAK; p21活化蛋白激酶抗体。
用Ani-PAK进行的HEK293提取物的Western印迹
图：将HEK293细胞提取物（30μg）用GloboZymes亲和纯化的Anti-PAK多克隆抗体进行Western印迹。该频带对应于PAK65（65 kDa）。
背景：p21-GTPase激活的蛋白激酶家族（PAK's）调节许多过程。这些过程包括细胞形态和细胞骨架组织，细胞运动，凋亡，神经元功能和细胞周期进程等。PAK与几种重要的调节蛋白相互作用，包括蛋白磷酸酶2A（PP2A），核糖体蛋白S6激酶，混合谱系激酶（MLK2）和SH3-SH2接头Nck。PAK的生理学靶标包括p38 MAP激酶，JNK，cRaf-1和SynapsinI。PAK的催化结构域通过半胱天冬酶依赖性途径在凋亡细胞中产生。与天然酶不同，催化域不受Rac或Cdc42的影响。
本网站将在规定时间内给予删除等相关处理。
