PP2A A Subunit
Source: Recombinant Human
Purity: > 90% by SDS-PAGE, apparent Mr ~ 65-kDa
Supplied: 1 μg in 50 μl of 50 mM Tis-HCl pH 7.0 buffer containing 0.1 mM phenylmethanesulfonyl fluoride, 14 mM β-mercaptoethanol, 1 mM EDTA and 50% glycerol. Maintain in aliquots at -70°C. Avoid repeated thawing.
Synonyms: Regulatory 65 kDa A Subunit of Protein Phosphatase 2A; PR65
Background: The A PR65 subunit of PP2A serves as a scaffolding molecule to coordinate the assembly of the catalytic (PP2AC) and variable B subunits. This generates functionally diverse heterotrimers of the phosphatase. The A subunit contains 15 tandemly-repeated HEAT sequences which may participate in its function as a scaffolding molecule. The A subunit also serves to regulate the substrate selectivity of PP2AC and allosterically modulates its polycation response. Association of recombinant A subunit with PP2AC is relatively tight (apparent KD ~ 85 pM) and leads to marked suppression of phosphatase activity with several phosphoproteins substrates. By contrast, suppression of PP2A activity is markedly less with several less-structured or smaller substrates such as phosphopeptides. The affinity of the A subunit for PP2AC is diminished by almost 100-fold following introduction of a glutamate at position 416. This indicates that the basic motif in this region of the protein may be important for its interaction with PP2AC. Some evidence indicates a role for the A subunit in disease. For example, at least four low frequency mutations in genes encoding the A subunit with altered affinity for PP2AC have been identified in several different human cancers. Additionally, overexpression of the A subunit in rat embryo fibroblasts leads to multinucleated cells.
References: Eur J Biochem 267: 5209 Eur J Biochem 248: 200
GloboZymes抗PAK
GloboZymes Anti-PAK是一种亲和纯化的多克隆兔抗体。它被生产为对应于人PAK1残基484-502的合成肽。该抗体可用于蛋白质印迹。例如，抗体可以与30μgHEK293细胞提取物以1：1000稀释度使用。除了PAK1，该抗体还显示出与PAK2的反应性。
同义词：Anti-p65PAK; 抗p68PAK; p21活化蛋白激酶抗体。
用Ani-PAK进行的HEK293提取物的Western印迹
图：将HEK293细胞提取物（30μg）用GloboZymes亲和纯化的Anti-PAK多克隆抗体进行Western印迹。该频带对应于PAK65（65 kDa）。
背景：p21-GTPase激活的蛋白激酶家族（PAK's）调节许多过程。这些过程包括细胞形态和细胞骨架组织，细胞运动，凋亡，神经元功能和细胞周期进程等。PAK与几种重要的调节蛋白相互作用，包括蛋白磷酸酶2A（PP2A），核糖体蛋白S6激酶，混合谱系激酶（MLK2）和SH3-SH2接头Nck。PAK的生理学靶标包括p38 MAP激酶，JNK，cRaf-1和SynapsinI。PAK的催化结构域通过半胱天冬酶依赖性途径在凋亡细胞中产生。与天然酶不同，催化域不受Rac或Cdc42的影响。
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