PP2C, Protein Phosphatase 2C
PP2C Source: Bovine brain
Purity: > 90% by SDS-PAGE, apparent Mr ~ 45-kDa
Supplied: 1 μg in 50 μl 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA and 50% glycerol.
Activity: ~ 1000 units/mg with casein as substrate. One unit is the amount of PP2C that releases 1 nmol of inorganic phosphate from 32P-labeled casein per min. Maintain preparations in aliquots at -70° C. Avoid repeated thawing.
Synonyms: Protein Phosphatase 2C
GloboZymes PP2C preparations are useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and to evaluate the effects of test substances on the activity of the phosphatase.
Background: Protein Phosphatase 2C (PP2C) is one of the four major cytoplasmic protein serine/threonine phosphatases present in mammalian cells. It is a Mg2+-dependent protein phosphatase with a molecular mass of approximately 42 – 45 kDa. It regulates numerous cellular processes. For example, PP2C is involved in cell cycle control and the reversal of stress-activated protein kinase cascades.
Figure: SDS-PAGE pattern of a purified preparation of PP2C. The gel was stained with Coomassie Blue. The position of marker proteins is indicated.
Reference: Biochem J 287: 1019
GloboZymes抗PAK
GloboZymes Anti-PAK是一种亲和纯化的多克隆兔抗体。它被生产为对应于人PAK1残基484-502的合成肽。该抗体可用于蛋白质印迹。例如，抗体可以与30μgHEK293细胞提取物以1：1000稀释度使用。除了PAK1，该抗体还显示出与PAK2的反应性。
同义词：Anti-p65PAK; 抗p68PAK; p21活化蛋白激酶抗体。
用Ani-PAK进行的HEK293提取物的Western印迹
图：将HEK293细胞提取物（30μg）用GloboZymes亲和纯化的Anti-PAK多克隆抗体进行Western印迹。该频带对应于PAK65（65 kDa）。
背景：p21-GTPase激活的蛋白激酶家族（PAK's）调节许多过程。这些过程包括细胞形态和细胞骨架组织，细胞运动，凋亡，神经元功能和细胞周期进程等。PAK与几种重要的调节蛋白相互作用，包括蛋白磷酸酶2A（PP2A），核糖体蛋白S6激酶，混合谱系激酶（MLK2）和SH3-SH2接头Nck。PAK的生理学靶标包括p38 MAP激酶，JNK，cRaf-1和SynapsinI。PAK的催化结构域通过半胱天冬酶依赖性途径在凋亡细胞中产生。与天然酶不同，催化域不受Rac或Cdc42的影响。
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