PP2A2, Protein Phosphatase 2A2
Source: Bovine kidney
Purity: > 90% by SDS-PAGE, subunit apparent Mr’s ~ 36- and 65- kDa
Supplied: In 50 μl 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA and 50% glycerol.
Activity: ~ 2000 units/mg with myelin basic protein (MBP) as substrate. One unit is the amount of PP2A2 that releases 1 nmol of inorganic phosphate from 32P-labeled MBP per min. Maintain preparations in aliquots at -70° C. Avoid repeated thawing.
Synonyms: Protein Phosphatase 2A2; PP2A2
Background: Protein phosphatase 2A (PP2A) is a divalent cation-independent multifunctional protein serine threonine phosphatase. PP2A regulates numerous cellular processes including the cell cycle, growth and differentiation. Physiological targets of PP2A include cell surface receptors and ion channels, and protein kinases involved in mitogenic signaling and the cell cycle. PP2A also acts on numerous transcription factors as well as key regulatory enzymes in metabolism. PP2A is a growth and tumor suppressor. It is inhibited potently by tumor promoters such as okadaic acid. It is also inhibited potently by two cancer-associated cellular proteins, I1PP2A and I2PP2A/SET. In addition, the SV40 small t antigen replaces the B subunit of PP2A during viral transformation. This subverts the physiological function of a subset of PP2A complexes in a substrate selective manner.
Figure: Purified preparation of bovine kidney PP2A2 was subjected to SDS-PAGE. The gel was stained with Coomassie Blue. The 65-kda A (top) and 36-kDa C (bottom) subunits are seen.
Protein Phosphatase 2A2 (PP2A2) is a dimeric form of PP2A. It contains A (65 kDa) and C (36 kDa) subunits but lacks the B subunit present in PP2A1. GloboZymes purified PP2A2 preparations are used to study enzyme kinetics and regulation. They are used to dephosphorylate target substrates and to evaluate the effects of test substances on the activity of the phosphatase. The preparations can also be used for determining the role of the missing B subunit of the enzyme.
References: Biochem J 287: 1019; Proc Natl Acad Sci USA 90: 2500
GloboZymes抗PAK
GloboZymes Anti-PAK是一种亲和纯化的多克隆兔抗体。它被生产为对应于人PAK1残基484-502的合成肽。该抗体可用于蛋白质印迹。例如，抗体可以与30μgHEK293细胞提取物以1：1000稀释度使用。除了PAK1，该抗体还显示出与PAK2的反应性。
同义词：Anti-p65PAK; 抗p68PAK; p21活化蛋白激酶抗体。
用Ani-PAK进行的HEK293提取物的Western印迹
图：将HEK293细胞提取物（30μg）用GloboZymes亲和纯化的Anti-PAK多克隆抗体进行Western印迹。该频带对应于PAK65（65 kDa）。
背景：p21-GTPase激活的蛋白激酶家族（PAK's）调节许多过程。这些过程包括细胞形态和细胞骨架组织，细胞运动，凋亡，神经元功能和细胞周期进程等。PAK与几种重要的调节蛋白相互作用，包括蛋白磷酸酶2A（PP2A），核糖体蛋白S6激酶，混合谱系激酶（MLK2）和SH3-SH2接头Nck。PAK的生理学靶标包括p38 MAP激酶，JNK，cRaf-1和SynapsinI。PAK的催化结构域通过半胱天冬酶依赖性途径在凋亡细胞中产生。与天然酶不同，催化域不受Rac或Cdc42的影响。
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